Plasmid

Part:BBa_K1416004:Design

Designed by: Jordan Monk, Colin Brown, Dennis Mishler   Group: iGEM14_Austin_Texas   (2014-10-17)


pFRY: a Reporter Plasmid for Measuring ncAA Incorporation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5940
    Illegal XbaI site found at 2474
    Illegal XbaI site found at 3764
    Illegal PstI site found at 1388
    Illegal PstI site found at 1490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5940
    Illegal NheI site found at 1618
    Illegal PstI site found at 1388
    Illegal PstI site found at 1490
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5940
    Illegal BamHI site found at 679
    Illegal BamHI site found at 1456
    Illegal XhoI site found at 1429
    Illegal XhoI site found at 5853
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5940
    Illegal XbaI site found at 2474
    Illegal XbaI site found at 3764
    Illegal PstI site found at 1388
    Illegal PstI site found at 1490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5940
    Illegal XbaI site found at 2474
    Illegal XbaI site found at 3764
    Illegal PstI site found at 1388
    Illegal PstI site found at 1490
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4054


Design Notes

Source

References

1. Wandrey et al, 2016, Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform